Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy
نویسندگان
چکیده
منابع مشابه
Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy.
Numerous experiments on CHO cells have shown that endosomes are composed of separate vesicular and tubulovesicular compartments, such as the sorting endosome, the recycling compartment, and the late endosome. However, Hopkins et al. (Nature 346, 335-339, 1990) have reported that endosomes in HEp2 human carcinoma cells form an extensive tubular reticulum. To resolve their observations with previ...
متن کاملQuantification of low density lipoprotein binding and cholesterol accumulation by single human fibroblasts using fluorescence microscopy.
Using fluorescence microscopy, we have quantified low density lipoprotein (LDL) binding by indirect immunofluorescence and cellular cholesterol with the fluorescent sterol-binding polyene, filipin, in individual cultured human fibroblasts from normal subjects and from patients with heterozygous and homozygous familial hypercholesterolemia. LDL binding by fibroblasts from heterozygous patients w...
متن کاملDistribution of receptors for transferrin and low density lipoprotein on the surface of giant HeLa cells.
Giant HeLa cells, having a spread diameter of about 200 micrometers, were briefly surface-labeled at 0 degree C with 125I-labeled transferrin, low density lipoprotein, anti-HeLa cell antibody, or concanavalin A. The cells were washed at 0 degree C, fixed, and autoradiographed. The distribution of grains when either anti-HeLa cell antibodies or concanavalin A was used was roughly as expected: th...
متن کاملAutomated quantification of keratocyte density by using confocal microscopy in vivo.
PURPOSE To compare keratocyte density determined by using confocal microscopy with keratocyte density determined in the same corneas by histology. METHODS Digital en face images of central corneas were recorded three times by using confocal microscopy in vivo in six New Zealand White rabbits. Bright objects (keratocyte nuclei) in the images were automatically identified by using a custom algo...
متن کاملEffect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells
Background: Vascular calcification is an important stage in atherosclerosis. During this stage, vascular smooth muscle cells (VSMC) synthesize many osteogenic factors such as osteonectin (encoded by SPARC). Oxidative stress plays a critical role in atherosclerosis progression, and its accumulation in the vascular wall stimulates the development of atherosclerosis and vascular calcification. The...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 1994
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.107.8.2177